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MHC Class II expression by human glioma cells after in vitro incubation with soluble antigens
Author(s) -
Cusimano G.,
Palladini G.,
Lauro G. M.
Publication year - 1990
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1990.tb00969.x
Subject(s) - antigen processing , antigen presentation , antigen , mhc class i , major histocompatibility complex , mhc class ii , biology , mhc restriction , transporter associated with antigen processing , microbiology and biotechnology , immunology , in vitro , cd74 , cd1 , glioma , immune system , cd8 , cancer research , t cell , biochemistry , interleukin 21
Recent studies have suggested that astrocytes share with the macrophages several properties in vitro , among which is the ability to express MHC Class II molecules and to present some antigens to syngeneic primed lymphocytes (MHC‐restricted presentation). It has been claimed in the literature that astrocytes cannot start the presentation and cannot express the related MHC Class II molecules if not previously stimulated with ± IFN. In this paper we report that 2 human GFAP + glioma cell lines, incubated in culture with various soluble antigens for at least 24 h, were able, in the absence of γIFN or of activated lymphocytes, to express the MHC Class II and to expose the antigens on their surfaces. Moreover, when a lysosomotropic agent such as chloroquine was added during the incubation, no MHC Class II expression was observed. This last datum suggests that in astrocytes, as is already known in macrophages, the processing of antigens and their assembling with MHC Class II molecules probably involves the lysosomal apparatus.