Premium
Search for a retrovirus in long‐term cultured cerebrospinal fluid cells and peripheral blood mononuclear cells from patients with multiple sclerosis
Author(s) -
Höllsberg P.,
MøllerLarsen A.,
Pedersen F. Skou,
Justesen J.,
Hansen H. J.,
Haahr S.
Publication year - 1989
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1989.tb03935.x
Subject(s) - peripheral blood mononuclear cell , multiple sclerosis , cerebrospinal fluid , retrovirus , cell culture , immunology , biology , cell , virology , dexamethasone , virus , pathology , medicine , microbiology and biotechnology , endocrinology , in vitro , biochemistry , genetics
Long‐term peripheral blood mononuclear cell (MNC) cultures stimulated with interleukin 2 (IL‐2) or IL‐2 + phytohemagglutinin were established from 33 multiple sclerosis (MS) patients, 9 with other neurological diseases (OND), and 24 normal controls (C). Cultures were analysed for growth characteristics, reverse transcriptase (RT) in the culture medium, 2′‐5′ oligoadenylate synthetase in the cells, and cell morphology. None of these paramenters differed in the MS group compared with the OND and C groups. Furthermore, 11 cerebrospinal fluid cell cultures were established without feeder ceils. Morphology studies of the cells and RT assays of the supernatants from these cultures were normal. Induction studies by dexamethasone and 2‐bromo‐5′‐deoxuridine in 2 of these cultures did not reveal any signs of a virus. The significance of these results for the retrovirus hypothesis is discussed.