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Inhibitory effect of calcium channel blockers on proliferation of human glioma cells in vitro
Author(s) -
KunertRadek J.,
Stepien H.,
Radek A.,
Lyson K.,
Pawlikowski M.
Publication year - 1989
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1989.tb03731.x
Subject(s) - nimodipine , verapamil , calcium channel , glioma , calcium , in vitro , pharmacology , voltage dependent calcium channel , chemistry , cell growth , calcium channel blocker , channel blocker , thymidine , inhibitory postsynaptic potential , biology , cancer research , endocrinology , biochemistry , organic chemistry
— The effects of 2 specific calcium channel blockers, verapamil and nimodipine, on the proliferation of human glioma tumour cells were investigated in vitro. Tumour tissues for primary cell cultures were obtained bioptically from 3 patients with the histopathological diagnosis of glioblastoma. The [ 3 H]‐thymidine incorporation into glioma tumour cells DNA was used as a sensitive index of the cell proliferation. It was found that verapamil (10 −4 ‐10 −5 M) and nimodipine (10 −4 ‐10 −6 M) significantly inhibited the [ 3 H]‐thymidine uptake in a dose‐related manner. The inhibitory effect of both calcium channel antagonists was reversed by stimultaneous addition of calcium chloride (5 × 10 −3 M). These results indicate that verapamil and nimodipine may exert an antiproliferative effect on glioma cells growth acting through a blokade of specific voltage‐dependent calcium channels.