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Enzyme‐linked immunosorbent assay for detection of antibodies to extractable muscle antigens in myasthenia gravis
Author(s) -
Viana V. S. T.,
Bueno C.,
Marchiori P. E.,
Oliveira R. M.,
Assis J. L.,
Scaff M.,
Cossermelli W.
Publication year - 1986
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1986.tb04606.x
Subject(s) - myasthenia gravis , antigen , antibody , thymoma , skeletal muscle , chemistry , enzyme , microbiology and biotechnology , immunology , medicine , chromatography , pathology , biochemistry , biology
ABSTRACT A purified citric acid soluble extract from human skeletal muscle (AEMA) and a phosphate‐buffered saline extract from rabbit muscle acetone powder (EMA) were used to coat polystyrene beads in an enzyme — linked immunosorbent assay (ELISA). From 54 patients with myasthenia gravis, positive results were observed in 14. Five of 6 patients with thymoma had high levels of antibodies. With the diagnostic difficulties in detecting small and medium‐sized thymoma, a sensitive assay for detection of antibodies to muscle antigen may be an important supplementary tool to detect tumors at early stages.