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Indole levels in human lumbar and ventricular cerebrospinal fluid and the effect of L‐tryptophan administration
Author(s) -
Koskiniemi M.,
Laakso J.,
Kuurne T.,
Laipio M.,
Härkönen M.
Publication year - 1985
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1985.tb03176.x
Subject(s) - cerebrospinal fluid , tryptophan , lumbar , serotonin , medicine , high performance liquid chromatography , 5 hydroxytryptophan , endocrinology , chemistry , indole test , anesthesia , chromatography , amino acid , surgery , biochemistry , receptor
– Levels of tryptophan (TRP), 5‐hydroxytryptophan (5‐HTP), 5‐hydroxy‐tryptamine (5‐HT), and 5‐hydroxyindoleacetic acid (5‐HIAA) in human lumbar and ventricular cerebrospinal fluid (CSF) were measured by reversed phase liquid chromatography (HPLC) with electrochemical detection. The levels of TRP ranged from 1593 to 4865 nmol/l in ventricular (VF) and from 1257 to 2557 nmol/l in lumbar CSF. The level of 5‐HTP varied from 1.1 to 68.9 nmol/l in VF and from 5.3 to 10.8 nmol/l in lumbar CSF; no previous reports of 5‐HTP levels in CSF exist. The serotonin level was 1.9–27.3 nmol/l in VF and 5.7–12.0 nmol/l in lumbar CSF. The levels of 5‐HIAA were considerbaly higher in VF than in lumbar fluid with respective means of 498 ± 52.4 nmol/l and 112 ± 15.6 nmol/l ( P < 0.001). An oral dose of 2 g L‐tryptophan significantly increased all indole levels except that of 5‐HT, both in patients with progressive myoclonus epilepsy and in controls.