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Monoamine metabolites in human cerebrospinal fluid. HPLC/ED method
Author(s) -
Mena M. A.,
Aguado E G.,
Yebenes J. G.
Publication year - 1984
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1984.tb07804.x
Subject(s) - homovanillic acid , cerebrospinal fluid , monoamine neurotransmitter , 5 hydroxyindoleacetic acid , serotonin , tryptophan , dopamine , medicine , chemistry , high performance liquid chromatography , endocrinology , anesthesia , chromatography , biochemistry , amino acid , receptor
— Catecholamines and indolealkylamines are of clinical interest in neurological and psychiatric disorders. We measured 3‐methoxy‐DOPA, 3‐methoxy‐4‐hydroxyphenylglycol, dihydroxyphenylacetic acid, tryptophan, 5‐hydroxyindoleacetic acid and homovanillic acid in human cerebrospinal fluid with a simple, sensitive, inexpensive, rapid and accurate procedure using high performance liquid chromatography coupled to an electrochemical detector. Patients with Parkinson's disease have a decrement in homovanillic acid that is reversed by treatment with L‐3,4‐dihydroxyphenylalanine. After this medication, 3‐methoxy‐DOPA is measurable in cerebrospinal fluid. Patients with depression show a decrease in serotonin turnover expressed by diminished 5‐hydroxyindoleacetic acid content in cerebrospinal fluid. Depressed patients also show low levels of tryptophan. Monoamine metabolites are augmented in patients with subarachnoid hemorrhage.