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Direct tissue isoelectric focusing of nervous system and muscle sections for detection of IgG patterns
Author(s) -
Olsson Tomas,
Link Hans,
Kostulas Vasilios,
Henriksson K. G.
Publication year - 1983
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1983.tb04564.x
Subject(s) - immunofixation , isoelectric focusing , pathology , central nervous system , multiple sclerosis , nervous system , antiserum , biopsy , peripheral nervous system , muscle biopsy , polymyositis , autopsy , immunoglobulin g , antibody , medicine , biology , immunology , neuroscience , biochemistry , monoclonal , monoclonal antibody , enzyme
‐ Agarose isoelectric focusing (IF) of 5–70 μm sections of frozen autopsy material (direct tissue IF; DTIF) from the central nervous system (CNS) and peripheral nervous system (PNS), and from muscle biopsy, followed by immunofixation with unlabeled or radiolabeled anti‐serum against human γ chains or IgG Fc fragments has been developed for analysis of IgG patterns. The DTIF technique followed by antiserum immunofixation is a promising tool for characterization of proteins in autopsy and biopsy materials from nervous and muscular systems. In multiple sclerosis brains, oligoclonal IgG bands were found which differed regarding number and mobility between the brains, and also between two different regions in single brains. For PNS, IgG patterns were easily detectable. In polymyositis muscle, no difference was found for IgG patterns when compared with normal muscle or serum.