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Cerebral microembolization in the rat: Changes in blood‐brain barrier permeability and cerebral blood flow as related to the degree of ischemia
Author(s) -
Beley Alain,
Edvinsson Lars,
Hardebo Jan Erik
Publication year - 1981
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1981.tb04391.x
Subject(s) - cerebral blood flow , medicine , blood–brain barrier , embolization , ischemia , internal carotid artery , anesthesia , blood flow , extravasation , evans blue , cerebral hemisphere , hemodynamics , cerebral edema , cardiology , central nervous system , pathology , surgery , radiology
Unilateral cerebral microembolism was performed in the rat by injecting calibrated, 50 μm in diameter, carbonized microspheres into the internal carotid artery. The events that follow brain ischemia due to cerebral embolization were studied by the analysis of the blood‐brain barrier (BBB) function, the degree of regional cerebral blood flow (CBF) and the development of brain edema. Two hours after embolization there was no change in the brain water content. The local CBF ( 14 C‐ethanol technique) was only reduced in the ipsilateral hemisphere. Twenty‐four hours after embolization the brain water content was increased significantly in the ipsilateral, but not in the contralateral hemisphere. Local CBF further decreased in the ipsilateral hemisphere and a reduction in flow was also observed in the contralateral hemisphere. Embolization led to an increase in the BBB permeability, analysed as regional penetrability of 3 H‐dextran and of Evans blue‐albumin complexes, which was restricted to the side of the injection of the microspheres.