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Proliferation of multiple sclerosis cerebrospinal fluid lymphocytes after stimulation with measles virus antigens
Author(s) -
Reunanen Mauri,
Salmi Aimo,
Ilonen Jorma,
Herva Elja
Publication year - 1980
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1980.tb03039.x
Subject(s) - multiple sclerosis , cerebrospinal fluid , antigen , measles virus , immunology , stimulation , virus , measles , lymphocyte , virology , in vitro , medicine , biology , pathology , vaccination , biochemistry
A method for measuring the proliferative response of cerebrospinal fluid (CSF) lymphocytes upon stimulation with mitogens and viral antigens has been developed. The number of lymphoid cells in DNA synthesis after in vitro stimulation is counted and the results expressed as a percentage of the total number of surviving lymphocytes. Preliminary results on four MS patients indicated that their CSF lymphocytes responded considerably more strongly to purified measles virus antigen than did corresponding peripheral blood lymphocytes (PBL). The responses to crude herpes virus antigen were at least as strong in PBL as in CSF lymphocyte cultures from the same patients. The CSF lymphocytes responded slightly stronger to phytohemagglutinin than PBL did.