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AMINO ACID INCORPORATION INTO BRAIN SUBCELLULAR FRACTIONS IN EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS
Author(s) -
BABITCH JOSEPH A.,
BLOMSTRAND CHRISTIAN,
HAMBERGER ANDERS
Publication year - 1975
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1975.tb07602.x
Subject(s) - encephalomyelitis , microsome , tris , amino acid , in vitro , biochemistry , leucine , polyacrylamide gel electrophoresis , chemistry , guinea pig , enzyme , mitochondrion , microbiology and biotechnology , biology , central nervous system , endocrinology
The in vitro protein synthetic capacity of brain slices from guinea‐pigs in the late stage (17–18 days post‐induction) of experimental allergic encephalomyelitis was increased over that of Freund's adjuvant injected controls, as determined by the rate of ( 14 C‐leucine incorporation into both tris‐soluble and tris‐insoluble proteins. All subcellular fractions prepared from incubated slices showed increased incorporation, with a crude nuclear fraction having the largest increase. Isolated brain mitochondria from EAE animals incorporated more amino acid into protein during the late stage of the disease, while isolated microsomes and “pH 5 enzymes” show decreased amino acid incorporation compared with controls in the late stage of EAE. Polyacrylamide gel electrophoresis of acidic, soluble proteins isolated from ( 3 J)‐leucine labeled nuclear or synaptosomal fractions revealed that increases of incorporation were generalized, and not restricted to a few proteins.