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CEREBROSPINAL FLUID PHOSPHOLIPIDS: Quantitative Determination of Fractionated Phospholipids and Normal Values in the Cerebrospinal Fluid
Author(s) -
Pedersen Holger Ejgil
Publication year - 1973
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1973.tb01336.x
Subject(s) - cerebrospinal fluid , sphingomyelin , phospholipid , chromatography , choline , phosphatidyl choline , chemistry , biochemistry , composition (language) , cholesterol , pathology , medicine , membrane , linguistics , philosophy
Cerebrospinal fluid (CSF) lipids were extracted, subjected to thin‐layer chromatography and quantitative determination of total and fractionated phospholipids. The methods were tested by analysis of serum and CSF pools. In CSF, sphingomyelin, choline phosphoglyceride and ethanolamine phosphoglyceride were isolated and quantitated while in serum further lyso‐choline phosphoglyceride was determined. In a “normal” material of 46 patients, with disorders which were not thought to influence the composition of CSF, the total phospholipid phosphorus concentration averaged 168 μMg per 1 CSF. Sphingomyelin, choline phosphoglyceride, and ethanolamine phosphoglyceride accounted for 25, 70 and 5 per cent respectively. The sum of the fractions comprised 93 per cent of total phospholipid phosphorus found. Grouping according to sex and age did not reveal significant differences. A significant correlation was present between CSF phospholipid and protein concentrations.