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CEREBROSPINAL FLUID CHOLESTEROL: Quantitative Analysis of Unesterified and Esterified Cholesterol and Normal Values in the Cerebrospinal Fluid
Author(s) -
Pedersen Holger Ejgil
Publication year - 1973
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1111/j.1600-0404.1973.tb01335.x
Subject(s) - cerebrospinal fluid , cholesterol , chemistry , acetic acid , medicine , endocrinology , chromatography , biochemistry
Cerebrospinal fluid (CSF) lipids were extracted and subjected to thin layer chromatography. The unesterified and esterified cholesterol was scraped off the plates and quantitated by a FeCl 3 colour reaction. Silica gel reduced the colour development. This reduction was diminished by rinsing the plates with glacial acetic acid before activation. In 47 control patients without disorders thought to affect the CSF composition, the mean CSF cholesterol concentration was 3.41 μg/ml of which 48 % was esterified. No significant differences were found in relation to sex and age. A significant correlation was found between CSF cholesterol and protein concentrations, and between the percentage of esterified cholesterol and the protein concentration.