Open AccessMyocardial regeneration by transplantation of modified endothelial progenitor cells expressing SDF ‐1 in a rat model
Author(s) -
Schuh Alexander,
Kroh Andreas,
Konschalla Simone,
Liehn Elisa A.,
Sobota Radoslav M.,
Biessen Erik AL.,
Bot Ilze,
Sönmez Tolga Taha,
Schober Andreas,
Marx Nikolaus,
Weber Christian,
Sasse Alexander
Publication year - 2012
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2012.01539.x
Subject(s) - progenitor cell , transplantation , myocardial infarction , stromal cell , endothelial progenitor cell , ligation , regeneration (biology) , medicine , neovascularization , stem cell , cardiology , cancer research , angiogenesis , biology , microbiology and biotechnology
Cell based therapy has been shown to attenuate myocardial dysfunction after myocardial infarction (MI) in different acute and chronic animal models. It has been further shown that stromal‐cell derived factor‐1α ( SDF ‐1α) facilitates proliferation and migration of endogenous progenitor cells into injured tissue. The aim of the present study was to investigate the role of exogenously applied and endogenously mobilized cells in a regenerative strategy for MI therapy. Lentivirally SDF ‐1α‐infected endothelial progenitor cells ( EPC s) were injected after 90 min. of ligation and reperfusion of the left anterior descending artery ( LAD ) intramyocardial and intracoronary using a new rodent catheter system. Eight weeks after transplantation, echocardiography and isolated heart studies revealed a significant improvement of LV function after intramyocardial application of lentiviral with SDF ‐1 infected EPC s compared to medium control. Intracoronary application of cells did not lead to significant differences compared to medium injected control hearts. Histology showed a significantly elevated rate of apoptotic cells and augmented proliferation after transplantation of EPC s and EPC s + SDF ‐1α in infarcted myocardium. In addition, a significant increased density of CD 31 + vessel structures, a lower collagen content and higher numbers of inflammatory cells after transplantation of SDF ‐1 transgenic cells were detectable. Intramyocardial application of lentiviral‐infected EPC s is associated with a significant improvement of myocardial function after infarction, in contrast to an intracoronary application. Histological results revealed a significant augmentation of neovascularization, lower collagen content, higher numbers of inflammatory cells and remarkable alterations of apoptotic/proliferative processes in infarcted areas after cell transplantation.