Open AccessSequential use of human‐derived medium supplements favours cardiovascular tissue engineering
Author(s) -
Vis Paul W. Riem,
Sluijter Joost P. G.,
SoekhradjSoechit R. Sarita,
van Herwerden Lex A.,
Kluin Jolanda,
Bouten Carlijn V. C.
Publication year - 2012
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2011.01351.x
Subject(s) - zymography , fetal bovine serum , tissue engineering , tissue culture , platelet lysate , contraction (grammar) , extracellular matrix , cell culture , matrix (chemical analysis) , andrology , protease , cell growth , matrix metalloproteinase , chemistry , immunology , microbiology and biotechnology , biology , cell , biochemistry , medicine , biomedical engineering , platelet , in vitro , endocrinology , enzyme , genetics , chromatography
For clinical application of tissue engineering strategies, the use of animal‐derived serum in culture medium is not recommended, because it can evoke immune responses in patients. We previously observed that human platelet‐lysate (PL) is favourable for cell expansion, but generates weaker tissue as compared to culture in foetal bovine serum (FBS). We investigated if human serum (HS) is a better human supplement to increase tissue strength. Cells were isolated from venous grafts of 10 patients and expanded in media supplemented with PL or HS, to determine proliferation rates and expression of genes related to collagen production and maturation. Zymography was used to assess protease expression. Collagen contraction assays were used as a two‐dimensional (2D) model for matrix contraction. As a prove of principle, 3D tissue culture and tensile testing was performed for two patients, to determine tissue strength. Cell proliferation was lower in HS‐supplemented medium than in PL medium. The HS cells produced less active matrix metallo‐proteinase 2 (MMP2) and showed increased matrix contraction as indicated by gel contraction assays and 3D‐tissue culture. Tensile testing showed increased strength for tissues cultured in HS when compared to PL. This effect was more pronounced if cells were sequentially cultured in PL, followed by tissue culture in HS. These data suggest that sequential use of PL and HS as substitutes for FBS in culture medium for cardiovascular tissue engineering results in improved cell proliferation and tissue mechanical properties, as compared to use of PL or HS apart.