Open AccessArrhythmia and sudden death associated with elevated cardiac chloride channel activity
Author(s) -
Ye L.,
Zhu W.,
Backx P. H.,
Cortez M. A.,
Wu J.,
Chow Y.H.,
Mckerlie C.,
Wang A.,
Tsui L.C.,
Gross G.J.,
Hu J.
Publication year - 2011
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2010.01243.x
Subject(s) - medicine , cystic fibrosis transmembrane conductance regulator , effective refractory period , sudden cardiac death , cardiac electrophysiology , electrophysiology , cardiology , electrical conduction system of the heart , intracardiac injection , chloride channel , potassium channel , ion channel , ventricular tachycardia , pharmacology , endocrinology , electrocardiography , biology , cystic fibrosis , microbiology and biotechnology , receptor
The identification and analysis of several cationic ion channels and their associated genes have greatly improved our understanding of the molecular and cellular mechanisms of cardiac arrhythmia. Our objective in this study was to examine the involvement of anionic ion channels in cardiac arrhythmia. We used a transgenic mouse model to overexpress the human cystic fibrosis transmembrane conductance regulator (CFTR) gene, which encodes a cAMP‐regulated chloride channel. We used RNase protection and in situ hybridization assays to determine the level of CFTR expression, and radiotelemetry and in vivo electrophysiological study in combination with pharmacological intervention to analyse the cardiac function. Cardiac CFTR overexpression leads to stress‐related sudden death in this model. In vivo intracardiac electrophysiological studies performed in anaesthetized mice showed no significant differences in baseline conduction parameters including atrial‐His bundle (AH) or His bundle‐ventricular (HV) conduction intervals, atrioventricular (AV) Wenckebach or 2:1 AV block cycle length and AV nodal functional refractory period. However, following isoproterenol administration, there was marked slowing of conduction parameters, including high‐grade AV block in transgenic mice, with non‐sustained ventricular tachycardia easily inducible using programmed stimulation or burst pacing. Our sudden death mouse model can be a valuable tool for investigation of the role of chloride channels in arrhythmogenesis and, potentially, for future evaluation of novel anti‐arrhythmic therapeutic strategies and pharmacological agents.