Open AccessIdentification of a novel antigen cross‐presenting cell type in spleen
Author(s) -
Tan Jonathan K. H.,
Quah Ben J. C.,
Griffiths Kristin L.,
Periasamy Pravin,
Hey YingYing,
O’Neill Helen C.
Publication year - 2011
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2010.01089.x
Subject(s) - cross presentation , dendritic cell , cd11c , antigen , biology , antigen presenting cell , antigen presentation , t cell , immune system , cd8 , spleen , immunology , myeloid , microbiology and biotechnology , phenotype , biochemistry , gene
Antigen‐presenting cells (APC), like dendritic cells (DC), are essential for T‐cell activation, leading to immunity or tolerance. Multiple DC subsets each play a unique role in the immune response. Here, a novel splenic dendritic‐like APC has been characterized in mice that has immune function and cell surface phenotype distinct from other, described DC subsets. These were identified as a cell type continuously produced in spleen long‐term cultures (LTC) and have an in vivo equivalent cell type in mice, namely ‘L‐DC’. This study characterizes LTC‐DC in terms of marker phenotype and function, and compares them with L‐DC and other known splenic DC and myeloid subsets. L‐DC display a myeloid dendritic‐like phenotype equivalent to LTC‐DC as CD11c lo CD11b hi MHC‐II − CD8α − cells, distinct by high accessibility and endocytic capacity for blood‐borne antigen. Both LTC‐DC and L‐DC have strong antigen cross‐presentation ability leading to strong activation of CD8 + T cells, particularly after exposure to lipopolysaccharide. However, they have weak ability to stimulate CD4 + T cells in antigen‐specific responses. Evidence is presented here for a novel DC type produced by in vitro haematopoiesis which has distinct antigen‐presenting potential and reflects a DC subset present also in vivo in spleen.