Open AccessMolecular characterization and functional properties of cardiomyocytes derived from human inducible pluripotent stem cells
Author(s) -
Germanguz Igal,
Sedan Oshra,
ZeeviLevin Naama,
Shtrichman Ronit,
Barak Efrat,
Ziskind Anna,
Eliyahu Sivan,
Meiry Gideon,
Amit Michal,
ItskovitzEldor Joseph,
Binah Ofer
Publication year - 2011
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2009.00996.x
Subject(s) - induced pluripotent stem cell , stem cell , microbiology and biotechnology , human induced pluripotent stem cells , characterization (materials science) , biology , computational biology , chemistry , embryonic stem cell , genetics , nanotechnology , gene , materials science
In view of the therapeutic potential of cardiomyocytes derived from induced pluripotent stem (iPS) cells (iPS‐derived cardiomyocytes), in the present study we investigated in iPS‐derived cardiomyocytes, the functional properties related to [Ca 2+ ] i handling and contraction, the contribution of the sarcoplasmic reticulum (SR) Ca 2+ release to contraction and the b‐adrenergic inotropic responsiveness. The two iPS clones investigated here were generated through infection of human foreskin fibroblasts (HFF) with retroviruses containing the four human genes: OCT4, Sox2, Klf4 and C‐Myc. Our major findings showed that iPS‐derived cardiomyocytes: ( i ) express cardiac specific RNA and proteins; ( ii ) exhibit negative force–frequency relations and mild (compared to adult) post‐rest potentiation; ( iii ) respond to ryanodine and caffeine, albeit less than adult cardiomyocytes, and express the SR‐Ca 2+ handling proteins ryanodine receptor and calsequestrin. Hence, this study demonstrates that in our cardiomyocytes clones differentiated from HFF‐derived iPS, the functional properties related to excitation–contraction coupling, resemble in part those of adult cardiomyocytes.