Open AccessDedifferentiation of human articular chondrocytes is associated with alterations in expression patterns of GDF‐5 and its receptors
Author(s) -
Schlegel Werner,
Albrecht Christian,
Eckl Peter,
Freudenthaler Harald,
Berger Angelika,
Vécsei Vilmos,
Marlovits Stefan
Publication year - 2009
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2009.00953.x
Subject(s) - noggin , microbiology and biotechnology , cartilage , biology , immunofluorescence , receptor , transplantation , synovial joint , embryogenesis , embryo , morphogenesis , immunology , anatomy , pathology , articular cartilage , bone morphogenetic protein , gene , antibody , medicine , osteoarthritis , genetics , alternative medicine
Human articular chondrocytes are expanded in monolayer culture in order to obtain sufficient cells for matrix‐associated cartilage transplantation. During this proliferation process, the cells change their shape as well as their expression profile. These changes resemble those that occur during embryogenesis, when the limb anlagen form the interzone that later develops the joint cleft. We analysed the expression profile of genes that are reportedly important for these changes during embryogenesis within the dedifferentiation process of adult articular chondrocytes. We found GDF‐5 , BMPR‐Ib and connexin 43 up‐regulated, as well as a down‐regulation of BMPR‐Ia and noggin . C onnexin 32 could not be detected in either native cartilage or in dedifferentiated cells. The newly synthesized proteins were detected by immunofluorescence. There is evidence from our results that dedifferentiated chondrocytes resemble the cells from the interzone in developing synovial joints.