Open AccessIn vivo and in vitro assessment of brain bioenergetics in aging rats
Author(s) -
Vančová Ol’ga,
Bačiak Ladislav,
Kašparová Svatava,
Kucharská Jarmila,
Palacios Hector H.,
Horecký Jaromír,
Aliev Gjumrakch
Publication year - 2010
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2009.00879.x
Subject(s) - phosphocreatine , bioenergetics , in vivo , oxidative stress , oxidative phosphorylation , creatine kinase , medicine , endocrinology , mitochondrion , creatine , catalase , adenosine triphosphate , biology , chemistry , biochemistry , energy metabolism , microbiology and biotechnology
Brain energy disorders can be present in aged men and animals. To this respect, the mitochondrial and free radical theory of aging postulates that age‐associated brain energy disorders are caused by an imbalance between pro‐ and anti‐oxidants that can result in oxidative stress. Our study was designed to investigate brain energy metabolism and the activity of endogenous antioxidants during their lifespan in male Wistar rats. In vivo brain bioenergetics were measured using 31 P nuclear magnetic resonance (NMR) spectroscopy and in vitro by polarographic analysis of mitochondrial oxidative phosphorylation. When compared to the young controls, a significant decrease of age‐dependent mitochondrial respiration and adenosine‐3‐phosphate (ATP) production measured in vitro correlated with significant reduction of forward creatine kinase reaction (kfor) and with an increase in phosphocreatine (PCr)/ATP, PCr/Pi and PME/ATP ratio measured in vivo . The levels of enzymatic antioxidants catalase, GPx and GST significantly decreased in the brain tissue as well as in the peripheral blood of aged rats. We suppose that mitochondrial dysfunction and oxidative inactivation of endogenous enzymes may participate in age‐related disorders of brain energy metabolism.