Open AccessRegression and persistence: remodelling in a tissue engineered axial vascular assembly
Author(s) -
Polykandriotis E.,
Euler S.,
Arkudas A.,
Pryymachuk G.,
Beier J. P.,
Greil P.,
Dragu A.,
Lametschwandtner A.,
Kneser U.,
Horch R. E.
Publication year - 2009
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2009.00828.x
Subject(s) - persistence (discontinuity) , regression , vascular remodelling in the embryo , biology , medicine , pathology , geology , mathematics , statistics , geotechnical engineering
In later stages of vasculoangiogenesis a vascular network is going through a metamorphosis for optimal perfusion and economy of energy. In this study we make a quantitative approach to phenomena of remodelling in a bioartificial neovascular network and suggest variance of calibre as a parameter of neovascular maturation. For this study, 18 male Lewis rats were subjected to the AV loop operation in combination with a hard porous biogenic matrix and an isolation chamber. The animals were allocated into three groups for different explantation intervals set to 2, 4 and 8 weeks, respectively. Collective attributes like vascular density, percent fractional area and variance of calibre were evaluated for a predefined region of interest (ROI). Late morphogenesis was evaluated by means of scanning electron microscopy. After the fourth week the absolute number of vessels within the ROI decreased ( P < 0.03) whereas, on the contrary, the fractional area of all segments increased ( P < 0.02). The variance in calibre was significantly increased in the 8‐week group ( P < 0.05). Lymphatic growth after week 4, early pericyte migration as well as intussusceptive angiogenesis were identified immunohistologically. Phenomena of remodelling were evaluated quantitatively in a neovascular network and variance could be proposed as a parameter of net vascular maturation.