Open AccessCD34 + cells augment endothelial cell differentiation of CD14 + endothelial progenitor cells in vitro
Author(s) -
Krenning G.,
Strate B.W.A. van der,
Schipper M.,
van Seijen X.J. Gallego y,
Fernandes B.C.A.,
van Luyn M.J.A.,
Harmsen M.C.
Publication year - 2009
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2008.00479.x
Subject(s) - endothelial stem cell , progenitor cell , cd34 , biology , microbiology and biotechnology , endothelial progenitor cell , angiogenesis , stem cell , immunology , cancer research , in vitro , biochemistry
Neovascularization by endothelial progenitor cells (EPC) for the treatment of ischaemic diseases has been a topic of intense research. The CD34 + cell is often designated as EPC, because it contributes to repair of ischaemic injuries through neovascularization. However, incorporation of CD34 + cells into the neovasculature is limited, suggesting another role which could be paracrine. CD14 + cells can also differentiate into endothelial cells and contribute to neovascularization. However, the low proliferative capacity of CD14 + cell‐derived endothelial cells hampers their use as therapeutic cells. We made the assumption that an interaction between CD34 + and CD14 + cells augments endothelial differentiation of the CD14 + cells. In vitro , the influence of CD34 + cells on the endothelial differentiation capacity of CD14 + cells was investigated. Endothelial differentiation was analysed by expression of endothelial cell markers CD31, CD144, von Willebrand Factor and endothelial Nitric Oxide Synthase . Furthermore, we assessed proliferative capacity and endothelial cell function of the cells in culture. In monocultures, 63% of the CD14 + ‐derived cells adopted an endothelial cell phenotype, whereas in CD34 + /CD14 + co‐cultures 95% of the cells showed endothelial cell differentiation. Proliferation increased up to 12% in the CD34 + /CD14 + co‐cultures compared to both monocultures. CD34‐conditioned medium also increased endothelial differentiation of CD14 + cells. This effect was abrogated by hepatocyte growth factor neutralizing antibodies, but not by interleukin‐8 and monocyte chemoattractant protein‐1 neutralizing antibodies. We show that co‐culturing of CD34 + and CD14 + cells results in a proliferating population of functional endothelial cells, which may be suitable for treatment of ischaemic diseases such as myocardial infarction.