Open AccessE2F1 represses β‐catenin/TCF activity by direct up‐regulation of Siah1
Author(s) -
Xie Wei,
Jin Lei,
Mei Yide,
Wu Mian
Publication year - 2009
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2008.00423.x
Subject(s) - e2f1 , chromatin immunoprecipitation , psychological repression , biology , e2f , transcription factor , microbiology and biotechnology , ectopic expression , transcription (linguistics) , small hairpin rna , cancer research , promoter , gene expression , apoptosis , cell culture , gene , gene knockdown , genetics , linguistics , philosophy
Transcription factor E2F1 is a key regulator of cell proliferation and apoptosis. Its activity is strictly controlled by the pRB/E2F pathway. In the majority of cancer cells, however, this pathway is frequently found deregulated, and the underlying mechanism involving transcriptional control by E2F1 has not yet been fully elucidated. Here we report the identification of two putative E2F1‐binding sites located upstream from Siah1 transcription start site (+1). Chromatin immunoprecipitation assay reveals that transcription factor E2F1 is capable of binding to the putative sites, and luciferase reporter assay shows that E2F1 can activate transcription from the Siah1 promoter. Ectopic expression of E2F1 elevates the Siah1 level, hence suppressing the β‐catenin/TCF activity. Consistently, knock‐down of endogenous E2F1 by a shRNA strategy results in reduced expression of Siah1. Moreover, repression of β‐catenin/TCF activity by E2F1 can be attenuated by shRNA‐based repression of endogenous Siah1, implying that Siah1 is a bona fide E2F1 target gene, which at least partly, mediates the suppression of β‐catenin/TCF signalling pathway.