Open AccessIntravital multi‐photon microscopy reveals several levels of heterogeneity in endocytic uptake by mouse renal proximal tubules
Author(s) -
Caplanusi A.,
Parreira K.S.,
Lima W. Rezende,
Marien B.,
Van Der Smissen P.,
De Diesbach P.,
Devuyst O.,
Courtoy P.J.
Publication year - 2007
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2007.00192.x
Subject(s) - endocytic cycle , two photon excitation microscopy , fluorescence microscope , microscopy , kidney , intravital microscopy , renal cortex , function (biology) , kidney cortex , microbiology and biotechnology , multiphoton fluorescence microscope , pathology , biology , in vivo , chemistry , biophysics , fluorescence , endocytosis , cell , optics , medicine , endocrinology , physics , biochemistry
Understanding renal function requires one to integrate the structural complexity of kidney nephrons and the dynamic nature of their cellular processes. Multi‐photon fluorescence microscopy is a state‐of‐the‐art imaging technique for in vivo analysis of kidney tubules structure and function in real time. This study presents visual evidence for several levels of heterogeneity of proximal tubular endocytic uptake in the superficial renal mouse cortex and illustrates the potential of multi‐photon microscopy for providing a comprehensive and dynamic portrayal of renal function.