Open AccessIn vitro differentiation of human mesenchymal stem cells to epithelial lineage
Author(s) -
Păunescu Virgil,
Deak Erika,
Herman Diana,
Siska Ioana Raluca,
T˘anasie Gabriela,
Bunu Carmen,
Anghel Simona,
Tatu Calin A.,
Oprea Tudor I.,
Henschler Reinhard,
Rüster Brigitte,
Bistrian Roxana,
Seifried Erhard
Publication year - 2007
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2007.00041.x
Subject(s) - cytokeratin , hepatocyte growth factor , biology , mesenchymal stem cell , keratinocyte growth factor , microbiology and biotechnology , stem cell , amniotic stem cells , cellular differentiation , pathology , bone marrow , epidermal growth factor , stem cell transplantation for articular cartilage repair , clinical uses of mesenchymal stem cells , growth factor , immunocytochemistry , lineage markers , adult stem cell , in vitro , immunology , cell culture , endothelial stem cell , progenitor cell , immunohistochemistry , medicine , endocrinology , biochemistry , genetics , receptor , gene
Our study examined whether human bone marrow‐derived MSCs are able to differentiate, in vitro , into functional epithelial‐like cells. MSCs were isolated from the sternum of 8 patients with different hematological disorders. The surface phenotype of these cells was characterized.To induce epithelial differentiation, MSCs were cultured using Epidermal Growth Factor, Keratinocyte Growth Factor, Hepatocyte Growth Factor and Insulin‐like growth Factor‐II. Differentiated cells were further characterized both morphologically and functionally by their capacity to express markers with specificity for epithelial lineage. The expression of cytokeratin 19 was assessed by immunocytochemistry, and cytokeratin 18 was evaluated by quantitative RT‐PCR (Taq‐man). The data demonstrate that human MSCs isolated from human bone marrow can differentiate into epithelial‐like cells and may thus serve as a cell source for tissue engineering and cell therapy of epithelial tissue.