Open AccessGene transfer strategies in tissue engineering
Author(s) -
Bleiziffer Oliver,
Eriksson Elof,
Yao Feng,
Horch Raymund E.,
Kneser Ulrich
Publication year - 2007
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2007.00027.x
Subject(s) - tissue engineering , regenerative medicine , gene transfer , regeneration (biology) , gene delivery , genetic enhancement , cartilage , gene , biology , computational biology , bioinformatics , biomedical engineering , medicine , anatomy , stem cell , microbiology and biotechnology , genetics
• Introduction • Methods of gene delivery ‐ Non‐viral techniques ‐ Naked DNA ‐ Cationic liposomes‐ Viral techniques ‐ Retrovirus ‐ Adenovirus ‐ Adeno‐associated virus ‐ Herpes simplex virus• Gene delivery from scaffolds for tissue engineering • Gene therapy applications in tissue engineering ‐ Skin and wound healing ‐ Cartilage ‐ Bone ‐ Nerve ‐ Liver and endocrine pancreas ‐ Blood vessels• OutlookAbstract Aiming for regeneration of severed or lost parts of the body, the combined application of gene therapy and tissue engineering has received much attention by regenerative medicine. Techniques of molecular biology can enhance the regenerative potential of a biomaterial by co‐delivery of therapeutic genes, and several different strategies have been used to achieve that goal. Possibilities for application are many‐fold and have been investigated to regenerate tissues such as skin, cartilage, bone, nerve, liver, pancreas and blood vessels. This review discusses advantages and problems encountered with the different gene delivery strategies as far as they relate to tissue engineering, analyses the positive aspects of polymeric gene delivery from matrices and discusses advances and future challenges of gene transfer strategies in selected tissues.