Open AccessAutonomously vascularized cellular constructs in tissue engineering: opening a new perspective for biomedical science
Author(s) -
Polykandriotis E.,
Arkudas A.,
Horch R.E.,
Stürzl M.,
Kneser U.
Publication year - 2007
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2007.00012.x
Subject(s) - perspective (graphical) , tissue engineering , computer science , biology , microbiology and biotechnology , computational biology , data science , biomedical engineering , engineering , artificial intelligence
• Introduction • Cellular homeostasis and vascularization • Importance of vascularization: historical review • Multicellular implants in vivo• Models of axial vascular induction • Development of the AV loop model • Angiogenesis in multicellular implants in vivo : authors' experience – Surgical technique – Chamber – Soft matrices – Modulation – Solid matrices – Cell seeding – Evaluation methods• Physiology of angiogenesis in the AV loop – Flow dynamics and morphology – Molecular biology of angiogenesis in the loop• Axially vascularized constructs as tools for research – Application in basic biomedical science Recombinant cell populations Modulation of angiogenesis Hematogenous metastasis of tumours Recombinant cell populations Modulation of angiogenesis Hematogenous metastasis of tumours Hematogenous metastasis of tumours– Applications in reparative medicine Multicellular implants Vascular grafts and bio‐artificial vessels Multicellular implants Vascular grafts and bio‐artificial vessels• ConclusionAbstract In tissue engineering cell cultures play a crucial role besides the matrix materials for the end of substituting lost tissue functions. The cell itself is situated at the cross‐roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology. Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past. Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells. However, issues of vascularization in vivo limit transferability of these observations and restrict upscaling into clinical applications. Novel in vivo models of vascularization have evolved inspired from reconstructive microsurgical concepts and they encompass axial neovascularization by means of vascular induction. This work represents a brief description of latest developments and potential applications of neovascularization and angiogenesis in tissue engineering.