Open AccessA two‐dimensional electrophoresis preliminary approach to human hepatocarcinoma differentiation induced by PPAR‐agonists
Author(s) -
Bottoni Patrizia,
Giardina B.,
Martorana G. E.,
Zuppi Cecilia,
Sole P. De,
Rossi Cristina,
Scatena R.
Publication year - 2005
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2005.tb00371.x
Subject(s) - electrophoresis , peroxisome proliferator activated receptor , chemistry , microbiology and biotechnology , computational biology , biology , cancer research , medicine , receptor , biochemistry
Adopting biochemical and proteomic approaches, we investigated the effect of some PPAR‐agonists, a new class of differentiating agents, on human hepatocellular carcinoma Hep‐G2 cell line. Cancer differentiation was assayed by checking albumin, transferrin and α‐fetoprotein synthesis. Cell metabolism was studied by NMR spectroscopy of cell culture supernatants and by evaluation of mitochondrial respiratory chain enzyme activities. The two dimensional electrophoresis approach was employed to analyze modifications in the expression of cellular proteins linked to cell phenotype differentiation in the attempt to identify potential diagnostic and prognostic biomarkers of hepatocellular carcinoma. Results indicate that PPAR‐agonists are able to act as differentiating inducers in human hepatocellular carcinoma Hep‐G2 cell line as well as to inhibit mitochondrial respiratory chain Complex I, provoking a selective derangement of cellular oxidative metabolism. Lastly, two dimensional electrophoresis showed interesting modifications in the pattern of expression of cellular proteins that confirm biochemical data (increase in albumin and transferrin, decrease of alpha ‐fetoprotein synthesis) and, moreover, emphasize the meaning of these data by the increase of spots indicatively ascribed to HSP70 and catalase.