Open AccessDirect interaction between SNAP‐23 and L‐type Ca 2+ channel
Author(s) -
Cho Won Jin,
Jeremic Aleksandar,
Jena Bhanu P.
Publication year - 2005
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2005.tb00363.x
Subject(s) - syntaxin , secretion , chemistry , vesicle , immunoprecipitation , biophysics , membrane , microbiology and biotechnology , biology , biochemistry , gene
During secretion, membrane‐bound secretory vesicles dock and fuse at the base of porosomes in the cell plasma membrane. Among other proteins, the porosome is composed of SNAREs and Ca 2+ ‐channels. Ca 2+ ‐channels and SNAREs have been implicated in cell secretion. Several immunoprecipitation and binding studies suggest the physical interaction of the t‐SNARE proteins, Syntaxin‐1 and SNAP‐25 with various Ca 2+ ‐channels. In this study, using yeast two‐hybrid and immunoanalysis, we demonstrate for the first time, direct interaction of SNAP‐23 and a L‐type Ca 2+ ‐channel at the plasma membrane in pancreas.