Open AccessEndothelial cells from hematopoietic stem cells are functionally different from those of human umbilical vein
Author(s) -
Paunescu V.,
Suciu Erika,
Tatu C.,
Plesa Adriana,
Herman Diana,
Siska Ioana Raluca,
Suciu C.,
Crisnic Daniela,
Nistor Daciana,
Tanasie Gabriela,
Bunu Carmen,
Raica M.
Publication year - 2003
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2003.tb00248.x
Subject(s) - cd34 , umbilical vein , microbiology and biotechnology , endothelial stem cell , vasculogenesis , haematopoiesis , stem cell , biology , cd31 , cd40 , immunology , angiogenesis , progenitor cell , in vitro , cytotoxic t cell , cancer research , biochemistry
Hematopoietic stem cells have a remarkable plastic capacity, which allows them to differentiate into various cells, such as immune cells, nervous cells, muscle cells, bone and cartilaginous cells. The aim of this study was to show the capacity of stem cells to differentiate into endothelial cells, in culture, after addition of endothelial cells growth suplement (ECGS). We also compared the behavior of these cells with that of endothelial cells obtained from human umbilical vein (HUVEC). CD34+ cells obtained by immunomagnetic separation from human umbilical cord and placental blood were used. After 12‐15 days of culture in a medium containing ECGS, the cells showed morphological changes characteristic to endothelial cells and immunocytochemical analysis revealed the presence of CD31 surface antigen and von Willebrand factor. The flow‐cytometric analysis of endothelial cells adhesion molecules (ECAM) showed that endothelial cells derived from CD34+ cells expressed CD54/ICAM‐1 9.65 ± 0.2% and CD106/VCAM 7.73±0.3%, values similar to those expressed by HUVECs. After TNF incubation, ECAM expression increased only in HUVECs. These data demonstrate that a fraction of circulating CD34+ cells may develop some endothelial cell characteristics when cultured with ECGS, but they are functionally different from HUVECs.