Open AccessEx vivo differentiation of umbilical cord blood progenitor cells in the presence of placental conditioned medium
Author(s) -
Chivu Mihaela,
Diaconu Carmen C.,
Brasoveanu Lorelei,
Alexiu Irina,
Bleotu Coralia,
Banceanu G.,
Miscalencu D.,
Cernescu C.
Publication year - 2002
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2002.tb00459.x
Subject(s) - haematopoiesis , progenitor cell , stem cell , biology , cord blood , cd34 , bone marrow , andrology , immunology , ex vivo , microbiology and biotechnology , cd14 , flow cytometry , in vivo , medicine
Hematopoetic stem cells (HSC) are the progenitors for the lympho‐hematopoietic system, with long lifespan and high proliferation potential. Transplantation of HSC from bone marrow or peripheral blood represents a standard therapy in severe hematological conditions. A possible alternative source of HSC is the umbilical cord blood, prepared by various separation procedures followed by expansion in cultures supplemented with hematopoietic growth factors. In order to check the effects of placental conditioned medium (PCM) from placental cells culture upon viability of HSC, we added plasma, PCM, dimetil sulfoxyde or hemin in HSC cultures. Flow cytometry or direct scoring of solid cultures using CD45+, CD34+, CD71+ and CD14+ fluorescent‐labeled monoclonal antibodies evaluated the effects upon cell proliferation and colony forming ability of HSC cultures, versus controls. PCM produced the highest proliferation, followed by plasma, DMSO and hemin. PCM improved the survival time and maintained a higher proportion of immature cells. PCM stimulates the differentiation towards myeloid lineage progenitor cells (>90% being CD45+), increasing the percentage of CD14+, granulocites /monocytes precursors. It is highly suggestive that PCM contains growth factors or cytokines, which regulate the development of HSC. Characterization of these factors is in progress.