Open AccessOxidative damage following cerebral ischemia depends on reperfusion ‐ a biochemical study in rat
Author(s) -
Nita D. Al.,
Nita Viorica,
Spulber St.,
Moldovan M.,
Popa Daniela Paula,
Zagrean AnaMaria,
Zagrean L.
Publication year - 2001
Publication title -
journal of cellular and molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.44
H-Index - 130
eISSN - 1582-4934
pISSN - 1582-1838
DOI - 10.1111/j.1582-4934.2001.tb00149.x
Subject(s) - ischemia , glutathione peroxidase , lipid peroxidation , chemistry , antioxidant , catalase , reperfusion injury , glutathione , dismutase , oxidative stress , superoxide dismutase , biochemistry , medicine , pharmacology , endocrinology , enzyme
The extent of brain injury during reperfusion appears to depend on the experimental pattern of ischemia/reperfusion. The goals of this study were: first, to identify the rate of free radicals generation and the antioxidant activity during ischemia and reperfusion by means of biochemical measurement of lipid peroxidation (LPO) and both enzymatic (superoxid dismutase ‐ SOD, catalase ‐ CAT, glutathion peroxidase ‐ GPx) and non‐enzymatic antioxidants activity (glutathione ‐ GSH); and second, to try to find out how the pattern of reperfusion may influence the balance between free radical production and clearance. Wistar male rats were subject of four‐vessel occlusion model (Pulsinelly & Brierley) cerebral blood flow being controlled by means of two atraumatic arterial microclamps placed on carotid arteries. The level of free radicals and the antioxidant activity were measured in ischemic rat brain tissue homogenate using spectrophotometrical techniques. All groups subjected to ischemia shown an increase of LPO and a reduction of the activity of enzymatic antioxidative systems (CAT, GPx, SOD) and non‐enzymatic systems (GSH). For both groups subjected to ischemia and reperfusion, results shown an important increase of LPO but less significant than the levels found in the group with ischemia only. Statistically relevant differences (p<0.01) between continuous reperfusion and fragmented reperfusion were observed concerning the LPO, CAT, SOD and GSH levels, oxidative aggresion during fragmented reperfusion being more important.