Open AccessDetoxification of aflatoxin B 1 by manganese peroxidase from the white‐rot fungus Phanerochaete sordida YK‐624
Author(s) -
Wang Jianqiao,
Ogata Makoto,
Hirai Hirofumi,
Kawagishi Hirokazu
Publication year - 2011
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2010.02158.x
Subject(s) - aflatoxin , phanerochaete , manganese peroxidase , chemistry , mycotoxin , peroxidase , carcinogen , chrysosporium , food science , microbiology and biotechnology , enzyme , biochemistry , biology
Aflatoxin B 1 (AFB 1 ) is a potent mycotoxin with mutagenic, carcinogenic, teratogenic, hepatotoxic, and immunosuppressive properties. In order to develop a bioremediation system for AFB 1 ‐contaminated foods by white‐rot fungi or ligninolytic enzymes, AFB 1 was treated with manganese peroxidase (MnP) from the white‐rot fungus Phanerochaete sordida YK‐624. AFB 1 was eliminated by MnP. The maximum elimination (86.0%) of AFB 1 was observed after 48 h in a reaction mixture containing 5 nkat of MnP. The addition of Tween 80 enhanced AFB 1 elimination. The elimination of AFB 1 by MnP considerably reduced its mutagenic activity in an umu test, and the treatment of AFB 1 by 20 nkat MnP reduced the mutagenic activity by 69.2%. 1 H‐NMR and HR‐ESI‐MS analysis suggested that AFB 1 is first oxidized to AFB 1 ‐8,9‐epoxide by MnP and then hydrolyzed to AFB 1 ‐8,9‐dihydrodiol. This is the first report that MnP can effectively remove the mutagenic activity of AFB 1 by converting it into AFB 1 ‐8,9‐dihydrodiol.