Open AccessDistinct DNA repair pathways involving RecA and nonhomologous end joining in Mycobacterium smegmatis
Author(s) -
KoryckaMachala Malgorzata,
Brzostek Anna,
Rozalska Sylwia,
RumijowskaGalewicz Anna,
Dziedzic Renata,
Bowater Richard,
Dziadek Jaroslaw
Publication year - 2006
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00199.x
Subject(s) - mycobacterium smegmatis , non homologous end joining , plasmid , dna repair , biology , dna , gene , genetics , dna damage , mitomycin c , homologous recombination , microbiology and biotechnology , mycobacterium tuberculosis , medicine , tuberculosis , pathology
Mycobacterium smegmatis was used to study the relationship between DNA repair processes involving RecA and nonhomologous end joining (NHEJ). The effect of gene deletions in recA and/or in two genes involved in NHEJ ( ku and ligD ) was tested on the ability of bacteria to join breaks in plasmids transformed into them and in their response to chemicals that damage DNA. The results provide in vivo evidence that only NHEJ is required for the repair of noncompatible DNA ends. By contrast, the response of mycobacteria to mitomycin C preferentially involved a RecA‐dependent pathway.