Open AccessThe cme gene of Clostridium difficile confers multidrug resistance in Enterococcus faecalis
Author(s) -
Lebel Sacha,
Bouttier Sylvie,
Lambert Thierry
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2004.tb09742.x
Subject(s) - orfs , enterococcus faecalis , microbiology and biotechnology , biology , efflux , clostridium difficile , staphylococcus aureus , shuttle vector , bacteria , enterococcus , gene , genetics , open reading frame , antibiotics , vector (molecular biology) , peptide sequence , recombinant dna
Antibiotic resistance in C. difficile by efflux has been previously suggested. The genome of C. difficile 630 was screened for sequences encoding putative proteins homologous to NorA from Staphylococcus aureus . Four ORFs homologous to efflux genes were cloned into the pAT79 shuttle vector under the control of transcription and translation signals of Gram‐positive bacteria and expressed in Enterococcus faecalis JH2‐2 and S. aureus RN4220. One of these sequences, designated cme conferred resistance to ethidium bromide, safranin O, and erythromycin in E. faecalis . The three other ORFs did not confer detectable resistance in both bacteria.