Premium
In vitro cultivation of parthenogenesis‐inducing Wolbachia in an Aedes albopictus cell line
Author(s) -
Kubota Masako,
Morii Tomohiro,
Miura Kazuki
Publication year - 2005
Publication title -
entomologia experimentalis et applicata
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 83
eISSN - 1570-7458
pISSN - 0013-8703
DOI - 10.1111/j.1570-7458.2005.00335.x
Subject(s) - wolbachia , biology , parthenogenesis , aedes albopictus , trichogrammatidae , parasitoid , hymenoptera , trichogramma , botany , zoology , genetics , gene , aedes aegypti , larva , embryo
Parthenogenesis‐inducing (PI) Wolbachia , infecting the parasitoid wasp Trichogramma kaykai Pinto and Stouthamer (Hymenoptera: Trichogrammatidae), were successfully maintained and cultivated in a mosquito, Aedes albopictus (Skuse) (Diptera: Culicidae), cell line, NIAS‐AeAl‐2. A parthenogenesis‐inducing (PI) Wolbachia strains with wasp ovaries were introduced into flasks with cultures of the cell line. The PI Wolbachia proliferated in NIAS‐AeAl‐2 as confirmed using a quantitative polymerase chain reaction method. Our final goal is to induce parthenogenesis in other hymenopteran species of commercial interest through the artificial transfer of PI Wolbachia . As a step towards this goal, we microinjected pupae of Trichogramma with PI Wolbachia culture in NIAS‐AeAl‐2 cells and PI Wolbachia was detected from recipient by PCR with Wolbachia specific primers ( wsp gene).