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‘Osmotic pump‘ feeding by coreids
Author(s) -
Miles P. W.,
Taylor G. S.
Publication year - 1994
Publication title -
entomologia experimentalis et applicata
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 83
eISSN - 1570-7458
pISSN - 0013-8703
DOI - 10.1111/j.1570-7458.1994.tb01852.x
Subject(s) - biology , fructose , sucrose , phloem , shoot , hindgut , carbohydrase , apoplast , invertase , plasmolysis , biochemistry , botany , glutamine , amino acid , enzyme , cell wall , midgut , larva
Species of Coreidae (Heteroptera) cause ‘water soaked’ lesions in their food plants. Such insects typically feed from parenchyma in and surrounding vascular tissues and also cause acropetal wilting and necrosis of small diameter shoots. Feeding by Mictis profana (Fabr.) in South Australia on the shoots of Acacia iteaphylla F. Muell. ex Benth. was found to cause a local, concurrent increase in both water content and free amino acid concentration, consistent with phloem unloading. Coreids, unlike other groups of phytophagous Heteroptera, secrete a salivary sucrase (α‐D‐glucohydrolase, EC 3.2.1.48) as probably the sole salivary carbohydrase, and tissues attacked by M. profana showed more sucrose hydrolysing activity than unattacked. The salivary enzyme is postulated to cause unloading of solutes into the apoplast due to the osmotic effects of conversion of endogenous sucrose to glucose and fructose, allowing the insect to suck the leaked contents of many cells from a single locus. The term ‘osmotic pump feeding’ is proposed for such a process. In demonstrations of its feasibility, infiltration of shoots with mixtures of glucose and fructose stoichiometrically equivalent to isosmotic sucrose increased the amounts of tissue sap and amino acid that could be sucked from the tissues; similarly, invertase and 1 M sugars forced into the extracellular space of stem sections increased the amino acids offloaded into the bathing solutions.