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TECHNIQUES FOR KEEPING WHEAT BULB FLY LARVAE ( LEPTOHYLEMYIA COARCTATA ) IN STERILE CONDITIONS
Author(s) -
HAMID S.
Publication year - 1966
Publication title -
entomologia experimentalis et applicata
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.765
H-Index - 83
eISSN - 1570-7458
pISSN - 0013-8703
DOI - 10.1111/j.1570-7458.1966.tb01008.x
Subject(s) - biology , bulb , suspension (topology) , horticulture , membrane filter , botany , filter paper , agronomy , chromatography , mathematics , chemistry , genetics , homotopy , membrane , pure mathematics
Three techniques are here described with special reference to investigation of bacterial diseases, as recently reported by H amid (1966). Wheat seed without chemical dressing is sown in boxes and allowed to grow 10 cm high. Plants are then lifted. After washing off soil and discarding the roots and apical half of the leaves, the central succulent part of the plant is chopped up with scissors into very fine pieces. These pieces are crushed in a basin and after adding sufficient water to give a thick suspension, finally macerated for about 5 minutes (a top‐drive macerator, Townson and Mercer Ltd. was used). This thick suspension is then centrifuged for 5 minutes at a speed of 5000 revolutions per minute and filtered through a Seitz filter. In the following techniques the filtrate is referred to as “nutrient solution” and the suspension as “macerated wheat”. Rigid bacteriological standards of sterility are maintained throughout the techniques.