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GENETICS OF DROUGHT ADAPTATION IN ARABIDOPSIS THALIANA II. QTL ANALYSIS OF A NEW MAPPING POPULATION, KAS‐1 × TSU‐1
Author(s) -
McKay John K.,
Richards James H.,
Nemali Krishna S.,
Sen Saunak,
MitchellOlds Thomas,
Boles Sandra,
Stahl Eli A.,
Wayne Tierney,
Juenger Thomas E.
Publication year - 2008
Publication title -
evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.84
H-Index - 199
eISSN - 1558-5646
pISSN - 0014-3820
DOI - 10.1111/j.1558-5646.2008.00474.x
Subject(s) - biology , arabidopsis thaliana , adaptation (eye) , quantitative trait locus , genetics , population , arabidopsis , population genetics , evolutionary biology , gene , mutant , demography , neuroscience , sociology
Despite compelling evidence that adaptation to local climate is common in plant populations, little is known about the evolutionary genetics of traits that contribute to climatic adaptation. A screen of natural accessions of Arabidopsis thaliana revealed Tsu‐1 and Kas‐1 to be opposite extremes for water‐use efficiency and climate at collection sites for these accessions differs greatly. To provide a tool to understand the genetic basis of this putative adaptation, Kas‐1 and Tsu‐1 were reciprocally crossed to create a new mapping population. Analysis of F 3 families showed segregating variation in both δ 13 C and transpiration rate, and as expected these traits had a negative genetic correlation ( r g =− 0.3). 346 RILs, 148 with Kas‐1 cytoplasm and 198 with Tsu‐1 cytoplasm, were advanced to the F 9 and genotyped using 48 microsatellites and 55 SNPs for a total of 103 markers. This mapping population was used for QTL analysis of δ 13 C using F 9 RIL means. Analysis of this reciprocal cross showed a large effect of cytoplasmic background, as well as two QTL for δ 13 C. The Kas‐1 × Tsu‐1 mapping population provides a powerful new resource for mapping QTL underlying natural variation and for dissecting the genetic basis of water‐use efficiency differences.