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TWO NEUTRALITY TESTS OF Y‐LINKED RDNA VARIATION IN DROSOPHILA MELANOGASTER
Author(s) -
Clark Andrew G.,
Lyckegaard Eva M. S.
Publication year - 1990
Publication title -
evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.84
H-Index - 199
eISSN - 1558-5646
pISSN - 0014-3820
DOI - 10.1111/j.1558-5646.1990.tb04315.x
Subject(s) - biology , genetics , restriction fragment length polymorphism , restriction enzyme , ribosomal dna , allele , melanogaster , ribosomal rna , drosophila melanogaster , microbiology and biotechnology , polymerase chain reaction , dna , gene , phylogenetics
The evolutionary significance of Y‐chromosomal ribosomal DNA sequence variation was tested by two different means. A single sample of males and females was collected from a peach orchard in central Pennsylvania. Wild‐caught males and sons of wild‐caught, wild‐inseminated females were crossed to virgin females having an X‐linked rDNA deficiency. Genomic DNA from male progeny of these crosses was extracted and digested with the single restriction endonuclease, Dra I. Southern blots of these digestions, when probed with the complete rDNA probe, revealed 10 distinct patterns of restriction fragments. A chisquare test for the homogeneity of the frequency distributions of the sample of wild males and sons of wild females failed to reject a neutral null hypothesis. The allele frequency configuration was tested with the Ewens‐Watterson test, and the departure from the infinite alleles neutral model was not significant. Simulations were performed to test the sensitivity of the tests to misclassification and to quantify the power of the two tests.