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VARIATION IN RIBOSOMAL DNA AMONG BIOLOGICAL SPECIES OF ARMILLARIA , A GENUS OF ROOT‐INFECTING FUNGI
Author(s) -
Anderson James B.,
Bailey Steven S.,
Pukkila Patricia J.
Publication year - 1989
Publication title -
evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.84
H-Index - 199
eISSN - 1558-5646
pISSN - 0014-3820
DOI - 10.1111/j.1558-5646.1989.tb02615.x
Subject(s) - biology , ribosomal dna , ribosomal rna , intergenic region , restriction enzyme , genetics , restriction fragment length polymorphism , restriction site , genus , armillaria , 18s ribosomal rna , spacer dna , botany , phylogenetics , gene , polymerase chain reaction , genome
Armillaria is a genus of root‐infecting fungi composed of several biological species in North America and Europe. To examine relatedness among biological species, ribosomal DNA (rDNA) from one isolate was cloned and rDNAs from 30 isolates were mapped for eight restriction enzymes. The positions of the large (26S) and small (18S) rRNA cistrons were found by Northern hybridizations of total cellular RNA with rDNA subclones and by alignment of maps with conserved restriction sites present in rRNA genes of other fungi. Nine restriction‐site and two length polymorphisms were observed. Eight North American (Roman numerals) and five European (species epithets) biological species could be placed in six classes with respect to rDNA maps (rDNA class 1: I and A. ostoyae ; class 2: II; class 3: A. borealis ; class 4: V, IX, and X; class 5: III, VII, A. lutea, and A. cepistipes ; and class 6: VI and A. mellea ). Most, but not all, polymorphisms were in intergenic regions.