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A Comparison of Chemical Enhancements for the Detection of Latent Blood , ,
Author(s) -
Seashols Sarah J.,
Cross Heather D.,
Shrader Danielle L.,
Rief Ashley
Publication year - 2013
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2012.02259.x
Subject(s) - luminol , human blood , blood stains , chromatography , serial dilution , chemistry , whole blood , chemiluminescence , reagent , medicine , organic chemistry , surgery , physiology , pathology , alternative medicine
Luminol, B luestar ® , and H emascein ® were tested to compare detection sensitivities to latent blood. Untreated, EDTA ‐treated human blood, and a catalytically similar blood substitute were diluted (neat to 1:1,000,000) and pipetted onto a variety of substrates. Luminol and B luestar ® performed similarly on all surfaces and fabrics. H emascein ® yielded poor results on wood surfaces, but performed well in the detection of latent blood on fabrics. Results from untreated, EDTA ‐treated, and synthetic blood results indicate that EDTA ‐treated blood is similar or slightly less sensitive than untreated blood at all dilutions and on all substrates, and the synthetic blood is less sensitive than real blood, but consistent in detection threshold and thus is useful as a training aid. Additionally, some foods and household chemicals that have previously been shown to cross‐react were tested with B luestar ® , H emascein ® , and luminol. H emascein ® cross‐reacted with many substances, while both luminol reagents were more discriminating.