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Specificity, Sensitivity, and Operability of RSID™‐Urine for Forensic Identification of Urine: Comparison with ELISA for Tamm‐Horsfall Protein
Author(s) -
Akutsu Tomoko,
Watanabe Ken,
Sakurada Koichi
Publication year - 2012
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2012.02174.x
Subject(s) - urine , identification (biology) , tamm–horsfall protein , chromatography , operability , sensitivity (control systems) , forensic identification , medicine , chemistry , biology , engineering , genetics , reliability engineering , botany , electronic engineering
In this study, the specificity, sensitivity, and operability of RSID™‐Urine, a new immunochromatographic test for urine identification, was evaluated and compared with ELISA detection of Tamm‐Horsfall protein (THP). Urine was successfully identified among other body fluids using RSID™‐Urine and ELISA detection of THP. The detection limit of RSID™‐Urine equated to 0.5 μL of urine; although the sensitivity of RSID™‐Urine may be lower than that of ELISA detection of THP, it is thought to be sufficient for application to casework samples. However, results from RSID™‐Urine must be interpreted with caution when the sample may have been contaminated with blood or vaginal fluid, because this might inhibit urine detection. The RSID™‐Urine assay can be performed in just 15 min by dropping the extracted sample onto the test cassette. Therefore, RSID™‐Urine should be an effective tool for the forensic identification of urine, in addition to ELISA detection of THP.