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The Use of Crossover Immunoelectrophoresis to Detect Human Blood Protein in Soil from an Ambush Scene in Kosovo * ,†
Author(s) -
Tuller Hugh,
Saunders Rebecca
Publication year - 2012
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2012.02070.x
Subject(s) - human blood , immunoelectrophoresis , forensic science , biology , medicine , chemistry , veterinary medicine , antibody , immunology , physiology
This study examines the survivability of human blood proteins in soils from a year and a half old ambush scene in Kosovo. A total of 72 soil samples were collected, a number of which were directly associated with bone fragments or bullet projectiles. The samples were examined using crossover immunoelectrophoresis (CIEP) to determine the presence of blood protein and species affiliation. Human blood proteins were identified in 44 of the 72 samples (61%) with the majority of the positive observations (29 of 44) found 0.0–4.5 cm below ground surface (65%). Chi‐squared and two‐sample difference of proportions tests confirmed significant differences between samples with and without associated physical evidence and the presence and depth of human blood proteins. While DNA has largely replaced immunological analysis in forensic analyses, our results suggest that in particular situations, CIEP may still be a valuable tool in criminology.