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A Population Genetic Database of Cat Breeds Developed in Coordination with a Domestic Cat STR Multiplex *
Author(s) -
MenottiRaymond Marilyn,
David Victor A.,
Weir Bruce S.,
O’Brien Stephen J.
Publication year - 2012
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2011.02040.x
Subject(s) - loss of heterozygosity , biology , breed , multiplex , genetics , microsatellite , locus (genetics) , population , str multiplex system , evolutionary biology , allele , gene , demography , sociology
Abstract: A simple tandem repeat (STR) PCR‐based typing system developed for the genetic individualization of domestic cat samples has been used to generate a population genetic database of domestic cat breeds. A panel of 10 tetranucleotide STR loci and a gender‐identifying sequence tagged site (STS) were co‐amplified in genomic DNA of 1043 individuals representing 38 cat breeds. The STR panel exhibits relatively high heterozygosity in cat breeds, with an average 10‐locus heterozygosity of 0.71, which represents an average of 38 breed‐specific heterozygosities for the 10‐member panel. When the entire set of breed individuals was analyzed as a single population, a heterozygosity of 0.87 was observed. Heterozygosities obtained for the 10 loci range from 0.72 to 0.96. The power for genetic individualization of domestic cat samples of the multiplex is high, with a probability of match ( p m ) of 6.2E‐14, using a conservative θ = 0.05.