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Sensitive Determination of Barbiturates in Biological Matrix by Capillary Electrophoresis Using Online Large‐Volume Sample Stacking *
Author(s) -
Fan LiuYin,
He Ting,
Tang YunYun,
Zhang Wei,
Song ChaoJin,
Zhao Xia,
Zhao XiaoYu,
Cao ChengXi
Publication year - 2012
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2011.02034.x
Subject(s) - capillary electrophoresis , secobarbital , chromatography , chemistry , stacking , volume (thermodynamics) , barbital , analytical chemistry (journal) , capillary electrochromatography , cartridge , detection limit , matrix (chemical analysis) , materials science , pentobarbital , anesthesia , medicine , physics , organic chemistry , quantum mechanics , metallurgy
In China, some forensic cases are caused by barbiturates. Thus, the determination of trace level barbiturates in body fluid is important for the poisoning investigation. In this study, an online large‐volume sample stacking (LVSS) with polarity switching in capillary electrophoresis (CE) was applied for the sensitive determination of barbiturates. This technique involves injecting a large volume of sample into a capillary and removing the sample matrix plug out of the capillary by reversing the polarity. Quantitation limit obtained was 0.048, 0.057, 0.039, and 0.015 μg/mL for secobarbital, amobarbital, barbital, and phenobarbital (signal‐to‐noise ratio = 9). By using LVSS, the stacking was simply achieved at 171.7‐, 169.7‐, 202.7‐, and 169.1‐fold for the above four barbiturates. The relative standard deviation values of intraday and interday were <2.11% and 4.69%, respectively. Recoveries were ranged from 83.7 to 105.2%. Finally, the trace analysis method was applied to the analysis of real forensic specimens and has achieved satisfactory results.