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Developmental Validation of RSID™‐Semen: A Lateral Flow Immunochromatographic Strip Test for the Forensic Detection of Human Semen *
Author(s) -
Old Jennifer,
Schweers Brett A.,
Boonlayangoor Pravat W.,
Fischer Brian,
Miller Kevin W. P.,
Reich Karl
Publication year - 2012
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2011.01968.x
Subject(s) - semen , prostatic acid phosphatase , biology , stain , andrology , acid phosphatase , staining , medicine , anatomy , biochemistry , genetics , enzyme
Tests for the identification of semen commonly involve the microscopic visualization of spermatozoa or assays for the presence of seminal markers such as acid phosphatase (AP) or prostate‐specific antigen (PSA). Here, we describe the rapid stain identification kit for the identification of semen (RSID™‐Semen), a lateral flow immunochromatographic strip test that uses two antihuman semenogelin monoclonal antibodies to detect the presence of semenogelin. The RSID™‐Semen strip is specific for human semen, detecting <2.5 nL of semen, and does not cross‐react with other human or nonhuman tissues tested. RSID™‐Semen is more sensitive with certain forensic evidence samples containing mixtures of vaginal secretions and semen than either of the commercially available PSA‐based forensic semen detection tests or tests that measure AP activity that were tested in parallel. The RSID™‐Semen kit also allows sampling a fraction of a questioned stain while retaining the majority of the sample for further processing through short tandem repeat analysis.