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Field Testing of Collection Cards for Cannabis sativa Samples With a Single Hexanucleotide DNA Marker * ,†
Author(s) -
Allgeier Lindsay,
Hemenway John,
Shirley Nicholas,
LaNier Tommy,
Coyle Heather Miller
Publication year - 2011
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2011.01818.x
Subject(s) - cannabis sativa , hashish , cannabis , biology , dna profiling , microbiology and biotechnology , genetics , medicine , dna , botany , psychiatry
The validity and feasibility of using DNA collection cards in the field for preservation and analysis of Cannabis sativa genotypes were investigated using a highly specific hexanucleotide marker. Collection cards were submitted to the National Marijuana Initiative, which selectively trained and managed the collection of specific types of samples from a variety of participating agencies. Samples collected at seizure sites included fresh marijuana leaf samples, dried “dispensary” samples, U.S. border seizures, and hashish. Using a standardized PCR kit with custom‐labeled oligonucleotide primers specific to marijuana, collection cards produced eight genotypes and 13 different alleles, extremely low baselines, and no cross‐reactivity with control plant species. Results were produced from all sample types with the exception of hashish. Plant DNA collection cards represent an easily implementable method for the genetic identification and relatedness of C. sativa street and grow site–seized samples with applications for databasing and market disruption.