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A Quantitative Method for Simultaneous Determination of 5‐Methoxy‐ N , N ‐Diisopropyltryptamine and its Metabolites in Urine Using Liquid Chromatography–Electrospray lonization–Tandem Mass Spectrometry
Author(s) -
Jin Ming J.,
Jin Changbae,
Kim Jin Y.,
In Moon K.,
Kwon Oh S.,
Yoo Hye H.
Publication year - 2011
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2011.01753.x
Subject(s) - chromatography , chemistry , formic acid , protein precipitation , detection limit , urine , tandem mass spectrometry , electrospray , selected reaction monitoring , liquid chromatography–mass spectrometry , elution , analyte , mass spectrometry , electrospray ionization , tryptamine , biochemistry
5‐Methoxy‐ N , N ‐diisopropyltryptamine (5‐MeO‐DIPT) is a designer hallucinogen derived from tryptamine and is reportedly abused and involved in criminal activities. For the detection of 5‐MeO‐DIPT use, a liquid chromatography–tandem mass spectrometric method for 5‐MeO‐DIPT and its metabolites, 5‐hydroxy‐ N , N ‐diisopropyltryptamine (5‐OH‐DIPT) and 5‐methoxy‐ N , N ‐isopropyltryptamine (5‐MeO‐IPT) was developed and validated in rat urine. The urine samples were pretreated by protein precipitation with acetonitrile and introduced into a BDS HYPERSIL C 18 column (50 × 2.0 mm, 5 μm) for chromatographic separation. Mobile phases consisted of methanol, water, and 1% formic acid, and gradient elution was used at a flow rate of 0.2 mL/min. For the MS detection, multiple‐reaction monitoring analysis was adopted. The linear range was 0.01–10 μg/mL, and the lower limit of quantification was 10 ng/mL for all analytes. The intra‐ and interday accuracies and precisions met the criteria (<15%). The developed method was successfully applied to the drug‐treated rat urine.