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Identification of the Source of Ivory Idol by DNA Analysis *
Author(s) -
Gupta Sandeep Kumar,
Thangaraj Kumarasamy,
Singh Lalji
Publication year - 2011
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2011.01750.x
Subject(s) - mitochondrial dna , dna extraction , dna , polymerase chain reaction , identification (biology) , biology , dna profiling , genetics , microbiology and biotechnology , gene , botany
In this study, we describe a forensic case dealing with the identification of the source of the processed ivory object by DNA analysis. Two pieces of Lord Krishna’s idols from a shop were confiscated by an investigating agency of the Indian government and forwarded to us to identify the source of its origin. We succeeded in isolating DNA from both processed ivory idols by using the phenol/chloroform DNA extraction method. The extracted DNA was subjected to PCR amplification using an elephant‐specific mitochondrial DNA (mtDNA) D‐loop marker. DNA sequence analysis of the amplified fragment of mtDNA D‐loop region confirmed that the idols were consistent with Asian elephant with 99% similarity.