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A New Strategy for the Discrimination of Mitochondrial DNA Haplogroups in Han Population
Author(s) -
Yang Yi,
Zhang Ping,
He Qiang,
Zhu Yiling,
Yang Xianghong,
Lv Rong,
Chen Jianghua
Publication year - 2011
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2011.01711.x
Subject(s) - haplogroup , mitochondrial dna , human mitochondrial dna haplogroup , genetics , population , haplotype , biology , evolutionary biology , criminology , computational biology , medicine , psychology , environmental health , gene , genotype
Mitochondrial DNA (mtDNA) haplogroup discrimination is interesting not only for phylogenetic and clinical but also for forensic studies. We discriminated the mtDNA haplogroups of 570 healthy unrelated Han people from Zhejiang Province, Southeast China, by comprehensive analysis mutations of the hypervariable segments‐I sequence and diagnostic polymorphisms in mtDNA coding region using real‐time polymerase chain reaction (RT‐PCR), which was compared with the widely used PCR and restriction fragment length polymorphism (PCR‐RFLP) method. The results showed that in superhaplogroup M, haplogroup D was the most common haplotype within this assay to 24.6%, and in the other superhaplotype N, haplogroup B and F were the most common groups. Samples re‐identified by PCR‐RFLP showed the consistent results that were got with RT‐PCR. In conclusion, the RT‐PCR strategy appears to be an accurate, reproducible, and sensitive technique for the discrimination of mtDNA haplogroups, especially for mass screenings quickly and economically.